Pyrophosphate Dependent Phosphofructokinase of Citrullus lanatus: Molecular Forms and Expression of Subunits
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چکیده
منابع مشابه
Pyrophosphate-dependent phosphofructokinase of Entamoeba histolytica: molecular cloning, recombinant expression and inhibition by pyrophosphate analogues.
By using oligonucleotide primers derived from regions highly conserved in prokaryotic and eukaryotic phosphofructokinase sequences, a genomic DNA fragment was amplified and used to isolate cDNA and genomic clones coding for PPi-dependent phosphofructokinase (PPi-PFK) of Entamoeba histolytica. The open reading frame consists of 1308 bp and the corresponding protein has a calculated molecular mas...
متن کاملMolecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon (Citrullus lanatus)
The complete mRNA sequence of watermelon Rab18 gene was amplified through the rapid amplification of cDNA ends (RACE) method. The full-length mRNA was 1010 bp containing a 645 bp open reading frame, which encodes a protein of 214 amino acids. Sequence analysis revealed that watermelon Rab18 protein shares high homology with the Rab18 of cucumber (99%), muskmelon (98%), Morus notabilis (90%), to...
متن کاملOrigin and emergence of the sweet dessert watermelon, Citrullus lanatus.
BACKGROUND AND AIMS Watermelons, Citrullus species (Cucurbitaceae), are native to Africa and have been cultivated since ancient times. The fruit flesh of wild watermelons is watery, but typically hard-textured, pale-coloured and bland or bitter. The familiar sweet dessert watermelons, C. lanatus, featuring non-bitter, tender, well-coloured flesh, have a narrow genetic base, suggesting that they...
متن کاملCloning, sequencing, and expression of pyrophosphate-dependent phosphofructokinase from Propionibacterium freudenreichii.
Pyrophosphate-dependent 6-phosphofructo-1-kinase (PPi-PFK) from Propionibacterium freudenreichii is a non-allosteric enzyme with properties dissimilar to those of other described phosphofructokinases. The enzyme was cloned into pBluescript, sequenced, and expressed in Escherichia coli at levels 15 times higher than those observed in Propionibacterium. The gene consists of 1215 bases which code ...
متن کاملFirst report on laxative activity of Citrullus lanatus
This study was aimed to assess the possible laxative effect of aqueous fruit pulp extract of Citrullus lanatus in albino’s Wistar rats. Rats were divided in 5 groups of 6 animals each, first group as control, second group served as standard (sodium picosulfate) while group 3, 4 and 5 were treated with fruit pulp aqueous fruit pulp extract of Citrullus lanatus at doses of 250, 500 and 1000 mg/kg...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1991
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.96.4.1185